Vaccination with Leptospiral Outer Membrane Lipoprotein LipL32 Reduces Kidney Invasion of Leptospira interrogans Serovar Canicola in Hamsters
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چکیده
منابع مشابه
Vaccination with leptospiral outer membrane lipoprotein LipL32 reduces kidney invasion of Leptospira interrogans serovar canicola in hamsters.
The Leptospira interrogans vaccines currently available are serovar specific and require regular booster immunizations to maintain protection of the host. In addition, a hamster challenge batch potency test is necessary to evaluate these vaccines prior to market release, requiring the use of a large number of animals, which is ethically and financially undesirable. Our previous work showed that...
متن کاملExpression and characterization of recombinant leptospiral outer membrane protein LipL32 from Leptospira interrogans serovar autumnalis.
Leptospira interrogans serovar autumnalis, a causative agent of leptospirosis in Thailand, was isolated from a patient for DNA extraction and amplification of LipL32 gene by polymerase chain reaction (PCR). The 782 bp PCR product was obtained, which was inserted into pAE plasmid with polyhistidine (His6 tag) to construct pAE-LipL32. This recombinant plasmid was transfected into E. coli BL21 (DE...
متن کاملImmunoreactive outer membrane proteins of Leptospira interrogans serovar Canicola strain Hond Utrecht IV.
BACKGROUND & OBJECTIVES Leptospirosis is a severe and complex zoonotic disease prevalent in many countries including India. Current leptospiral research is focussed on the identification of the outer membrane proteins (OMPs) of the organism that could be used in developing diagnostic assays for leptospirosis. METHODS The Leptospira interrogans serovar Canicola was grown in EMJH medium and the...
متن کاملEvaluation of recombinant Leptospira interrogans serovar canicola outer membrane proteins as diagnostic antigen.
Leptospirosis of animals and man is an important rereported in recent years are LipL32, LipL41 and OmpL1; they emerging infectious disease worldwide. In domestic animals are expressed in-vivo during infection. LipL32 and LipL41 it is an important cause of abortion, stillbirth, infertility, are important and surface-exposed leptospiral outer membrane decreased milk production and death. In dairy...
متن کاملamplification and cloning of a gene encoding a 41 kda outer membrane protein (lipl41) of leptospira interrogans serovar canicola
background: leptospirosis has been recognized as an important reemerging infectious disease caused by pathogenic leptospira spp. a major challenge of this disease is the application of a basic research to improve diagnostic method. outer membrane proteins of leptospira are potential candidates that could be useful in diagnosis. among them the lipl41 is an immunogenic protein which is present on...
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ژورنال
عنوان ژورنال: Clinical and Vaccine Immunology
سال: 2014
ISSN: 1556-6811,1556-679X
DOI: 10.1128/cvi.00719-13